crispri v4 vector (Addgene inc)
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crispri v4 vector
Crispri V4 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispri+vector/pLX303-ZIM3-KRAB-dCas9+(Plasmid+%23154472)/pm41759529-309-5-14
Average 95 stars, based on 32 article reviews
Crispri V4 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispri+vector/pLX303-ZIM3-KRAB-dCas9+(Plasmid+%23154472)/pm41759529-309-5-14
Average 95 stars, based on 32 article reviews
crispri v4 vector - by Bioz Stars,
2026-09
95/100 stars
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Polymerase Chain Reaction:Article Title: MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins Through the Action of the ERH Protein Article Snippet: To construct miR-144 hairpin variants (m1–5), we introduced mutations on primers that were used to reverse-PCR amplify and linearize the miR-144~451 plasmid; the linearized plasmids were then circularized by T4 Polynucleotide Kinase and T4 DNA ligase (NEB). .. To express miRNA hairpins under the U6 promoter, we PCR amplified corresponding miRNA hairpins and flanking sequences and cloned between BstXI and XhoI sites in the Amplification:Article Title: MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins Through the Action of the ERH Protein Article Snippet: To construct miR-144 hairpin variants (m1–5), we introduced mutations on primers that were used to reverse-PCR amplify and linearize the miR-144~451 plasmid; the linearized plasmids were then circularized by T4 Polynucleotide Kinase and T4 DNA ligase (NEB). .. To express miRNA hairpins under the U6 promoter, we PCR amplified corresponding miRNA hairpins and flanking sequences and cloned between BstXI and XhoI sites in the Clone Assay:Article Title: MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins Through the Action of the ERH Protein Article Snippet: To construct miR-144 hairpin variants (m1–5), we introduced mutations on primers that were used to reverse-PCR amplify and linearize the miR-144~451 plasmid; the linearized plasmids were then circularized by T4 Polynucleotide Kinase and T4 DNA ligase (NEB). .. To express miRNA hairpins under the U6 promoter, we PCR amplified corresponding miRNA hairpins and flanking sequences and cloned between BstXI and XhoI sites in the Plasmid Preparation:Article Title: MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins Through the Action of the ERH Protein Article Snippet: To construct miR-144 hairpin variants (m1–5), we introduced mutations on primers that were used to reverse-PCR amplify and linearize the miR-144~451 plasmid; the linearized plasmids were then circularized by T4 Polynucleotide Kinase and T4 DNA ligase (NEB). .. To express miRNA hairpins under the U6 promoter, we PCR amplified corresponding miRNA hairpins and flanking sequences and cloned between BstXI and XhoI sites in the Article Title: A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response Article Snippet: The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described CRISPRi vector (herein referred to as the “original sgRNA expression vector”) (Addgene, Cat#60955). .. The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described Article Title: A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response Article Snippet: Plasmid design and construction The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described CRISPRi vector (herein referred to as the “original sgRNA expression vector”) (Addgene, Cat#60955). .. Plasmid design and construction The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described other:Article Title: A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response Article Snippet: To construct pBA439, the mU6-sgRNA-EF1a-PURO-BFP region from this parental vector and a BGH polyadenylation sequence amplified by PCR from pcDNA3.1(+) (Invitrogen, V790-20) were inserted in reverse origination between the XbaI and EcoRI sites of the parental. Derivative Assay:Article Title: A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response Article Snippet: The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described CRISPRi vector (herein referred to as the “original sgRNA expression vector”) (Addgene, Cat#60955). .. The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described Article Title: A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response Article Snippet: Plasmid design and construction The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described CRISPRi vector (herein referred to as the “original sgRNA expression vector”) (Addgene, Cat#60955). .. Plasmid design and construction The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described Expressing:Article Title: A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response Article Snippet: The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described CRISPRi vector (herein referred to as the “original sgRNA expression vector”) (Addgene, Cat#60955). .. The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described Article Title: A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response Article Snippet: Plasmid design and construction The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described CRISPRi vector (herein referred to as the “original sgRNA expression vector”) (Addgene, Cat#60955). .. Plasmid design and construction The “Perturb-seq vector” backbone (pBA439, Addgene, Cat#85967) was derived from a previously described Transformation Assay:Article Title: Cell type-specific 3D epigenomes in the developing human cortex Article Snippet: .. For lentiviral packaging, the Article Title: 3D Epigenomic Characterization Reveals Insights Into Gene Regulation and Lineage Specification During Corticogenesis Article Snippet: .. For lentiviral packaging, the |
![A Left: Evolutionary tree of selected mammalian species. Right: Percent base conservation of human LPS-inducible macrophage lncRNA sequences across the genomes of indicated species, sorted by evolutionary distance; top panel includes all 24 lncRNAs from Fig. and data are shown as box plots (median and 75th-25th percentile interquartile range, whiskers indicate minimum and maximum data values), with species generation time overlayed; bottom panel focuses on specific lncRNAs. B Illustration of genomic locations of specified lncRNAs relative to nearest neighboring genes (distance in kilobases [kb] provided). Red triangles indicate transcriptional start site positions targeted by <t>CRISPRi</t> for lncRNA silencing. C Volcano plots from CRISPRi-based lncRNA loss-of-function experiments in THP1 cells stimulated with LPS for 8 h. Fold-changes (fc) compare lncRNA-knockdown cells to empty vector control cells. Results from three independent experiments. Two-tailed Student’s t-test p -values are shown. D Cytoscape network of lncRNAs (blue) and mRNAs from panel C , regulated upon lncRNA silencing (≥2-fold up or down, p ≤ 0.05, two-tailed Student’s t-test). E Pie charts showing proportions of all expressed mRNAs or LPS-responsive mRNAs (≥2-fold up or down, p ≤ 0.05, two-tailed Student’s t-test) affected by silencing of one or more lncRNAs. C – E: 8 h LPS-stimulated THP1 cells and three independent replicates.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5776/pmc12095776/pmc12095776__41467_2025_60084_Fig3_HTML.jpg)